Development of Chromatographic Fingerprint Profile and Multi-elemental Analysis of Datura metel L.

Pharmacognosy Research,2022,14,2,146-152.DOI:10.5530/pres.14.2.21Published:April 2022Type:Original ArticleAuthors:Shakila Ramachandran, Saravanan Meenatchisundaram, Subashini Subramanian, Sujith Thatipelli, Achintya Kumar Mandal, and Rajesh Allu Author(s) affiliations:Shakila Ramachandran1,*, Meenatchisundaram2, Subramanian3, Thatipelli4, Mandal5, Allu6 Department of Chemistry, Siddha Central Research Institute, Anna Hospital Campus, Arumbakkam, Chennai, Tamil Nadu, INDIA. Abstract:Background: The Solanaceae family member Datura metel L. has been related to therapeutic benefits. plant is widely used in Ayurveda systems Indian medicines. Objectives: Present study was focused understand the phytochemical multi elemental composition D. whole plant. Materials Methods: collected, shade dried, powdered subjected analysis physico-chemical parameters, thin layer chromatography, high performance chromatographic finger print profilings (HPTLC), pressure liquid profile at four wavelengths, X-ray fluorescence, energy dispersive analysis, photoelectron spectrometer, powder diffractometer inductively coupled plasma optical emission spectrometer. Results: In HPTLC, 7 spots under 254 nm, 10 366 nm 11 after derivatization with vanillin sulphuric acid reagent appeared. HPLC, 9 peaks 230 5 286 separated. Presence 17 elements such as copper, cobalt, nickel, manganese, zinc, iron, sodium, potassium, calcium, magnesium, phosphorus, aluminium, chlorine, carbon, oxygen, sulphur silicon were identified. Conclusion: K, Mg, Ca, Si, S Fe play vital role on human health. Chlorine, silicon, sulfur silica are first time identified which have significant biological functions. Key words: Ummattai, Devil’s trumpet, Solanaceae, Cardiarhythm, Asthma. View:PDF (605.77 KB)